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Part:BBa_K531007:Design

Designed by: Alexander Aaring   Group: iGEM11_Grinnell   (2011-09-21)


PrsaA constitutive promoter, esp optimized for Caulobacter, and rsaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 321
    Illegal XhoI site found at 891
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1146
    Illegal AgeI site found at 1269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Stop codon naturally occurring at the end of esp removed to ensure smooth expression of the fusion protein.


Source

PrsaA and rsaA C-terminal secretion tag both come from the genome of Caulobacter crescentus; esp optimized for Caulobacter crescentus is a synthetically codon optimized copy of esp as it is found in Staphylococcus epidermidis.

References